Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.
Identifieur interne : 000183 ( Main/Exploration ); précédent : 000182; suivant : 000184Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.
Auteurs : Salma Abdeljalil [Tunisie] ; Héla Trigui-Lahiani ; Houcine Lazzez ; Ali GargouriSource :
- Molecular biotechnology [ 1559-0305 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Alignement de séquences (MeSH), Banque de gènes (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Escherichia coli (métabolisme), Gènes fongiques (MeSH), Modèles moléculaires (MeSH), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéines recombinantes (composition chimique), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Stachybotrys (enzymologie), Stachybotrys (génétique), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Technique de Southern (MeSH), bêta-Glucosidase (composition chimique), bêta-Glucosidase (génétique), bêta-Glucosidase (métabolisme).
- MESH :
- composition chimique : Protéines fongiques, Protéines recombinantes, bêta-Glucosidase.
- enzymologie : Stachybotrys.
- génétique : ADN fongique, Escherichia coli, Protéines fongiques, Protéines recombinantes, Stachybotrys, bêta-Glucosidase.
- métabolisme : Escherichia coli, Protéines fongiques, Protéines recombinantes, bêta-Glucosidase.
- Alignement de séquences, Banque de gènes, Clonage moléculaire, Données de séquences moléculaires, Gènes fongiques, Modèles moléculaires, Séquence d'acides aminés, Séquence nucléotidique, Technique de Southern.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Blotting, Southern (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Escherichia coli (metabolism), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Library (MeSH), Genes, Fungal (MeSH), Models, Molecular (MeSH), Molecular Sequence Data (MeSH), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sequence Alignment (MeSH), Stachybotrys (enzymology), Stachybotrys (genetics), beta-Glucosidase (chemistry), beta-Glucosidase (genetics), beta-Glucosidase (metabolism).
- MESH :
- chemical , chemistry : Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , genetics : DNA, Fungal, Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- enzymology : Stachybotrys.
- genetics : Escherichia coli, Stachybotrys.
- metabolism : Escherichia coli, Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, Gene Library, Genes, Fungal, Models, Molecular, Molecular Sequence Data, Sequence Alignment.
Abstract
The filamentous fungus Stachybotrys microspora possess a rich β-glucosidase system composed of five β-glucosidases. Three of them were already purified to homogeneity and characterized. In order to isolate the β-glucosidase genes from S. microspora and study their regulation, a PCR strategy using consensus primers was used as a first step. This approach enabled the isolation of three different fragments of family 3 β-glucosidase gene. A representative genomic library was constructed and probed with one amplified fragment gene belonging to family 3 of β-glucosidase. After two rounds of hybridization, seven clones were obtained and the analysis of DNA plasmids leads to the isolation of one clone (CF3) with the largest insert of 7 kb. The regulatory region shows multiple TC-rich elements characteristic of constitutive promoter, explaining the expression of this gene under glucose condition, as shown by zymogram and RT-PCR analysis. The tertiary structure of the deduced amino acid sequence of Smbgl3 was predicted and has shown three conserved domains: an (α/β)8 triose phosphate isomerase (TIM) barrel, (α/β)5 sandwich, and fibronectin type III domain involved in protein thermostability. Zymogram analysis highlighted such thermostable character of this novel β-glucosidase.
DOI: 10.1007/s12033-012-9633-5
PubMed: 23242634
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000637
- to stream PubMed, to step Curation: 000633
- to stream PubMed, to step Checkpoint: 000629
- to stream Main, to step Merge: 000183
- to stream Main, to step Curation: 000183
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.</title><author><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax, Tunisia. salma.abdeljalil@gmail.com</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax</wicri:regionArea><wicri:noRegion>3018 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name></author><author><name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name></author><author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23242634</idno><idno type="pmid">23242634</idno><idno type="doi">10.1007/s12033-012-9633-5</idno><idno type="wicri:Area/PubMed/Corpus">000637</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000637</idno><idno type="wicri:Area/PubMed/Curation">000633</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000633</idno><idno type="wicri:Area/PubMed/Checkpoint">000629</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000629</idno><idno type="wicri:Area/Main/Merge">000183</idno><idno type="wicri:Area/Main/Curation">000183</idno><idno type="wicri:Area/Main/Exploration">000183</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.</title><author><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax, Tunisia. salma.abdeljalil@gmail.com</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax</wicri:regionArea><wicri:noRegion>3018 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name></author><author><name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name></author><author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></author></analytic><series><title level="j">Molecular biotechnology</title><idno type="eISSN">1559-0305</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Fungal (genetics)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Library (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Models, Molecular (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Stachybotrys (enzymology)</term><term>Stachybotrys (genetics)</term><term>beta-Glucosidase (chemistry)</term><term>beta-Glucosidase (genetics)</term><term>beta-Glucosidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Alignement de séquences (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Gènes fongiques (MeSH)</term><term>Modèles moléculaires (MeSH)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines recombinantes (composition chimique)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Stachybotrys (enzymologie)</term><term>Stachybotrys (génétique)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Technique de Southern (MeSH)</term><term>bêta-Glucosidase (composition chimique)</term><term>bêta-Glucosidase (génétique)</term><term>bêta-Glucosidase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term><term>Recombinant Proteins</term><term>beta-Glucosidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term><term>Fungal Proteins</term><term>Recombinant Proteins</term><term>beta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines fongiques</term><term>Protéines recombinantes</term><term>bêta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Stachybotrys</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Stachybotrys</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Stachybotrys</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Escherichia coli</term><term>Protéines fongiques</term><term>Protéines recombinantes</term><term>Stachybotrys</term><term>bêta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Fungal Proteins</term><term>Recombinant Proteins</term><term>beta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Protéines fongiques</term><term>Protéines recombinantes</term><term>bêta-Glucosidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Blotting, Southern</term><term>Cloning, Molecular</term><term>Gene Library</term><term>Genes, Fungal</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Banque de gènes</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Gènes fongiques</term><term>Modèles moléculaires</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Southern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The filamentous fungus Stachybotrys microspora possess a rich β-glucosidase system composed of five β-glucosidases. Three of them were already purified to homogeneity and characterized. In order to isolate the β-glucosidase genes from S. microspora and study their regulation, a PCR strategy using consensus primers was used as a first step. This approach enabled the isolation of three different fragments of family 3 β-glucosidase gene. A representative genomic library was constructed and probed with one amplified fragment gene belonging to family 3 of β-glucosidase. After two rounds of hybridization, seven clones were obtained and the analysis of DNA plasmids leads to the isolation of one clone (CF3) with the largest insert of 7 kb. The regulatory region shows multiple TC-rich elements characteristic of constitutive promoter, explaining the expression of this gene under glucose condition, as shown by zymogram and RT-PCR analysis. The tertiary structure of the deduced amino acid sequence of Smbgl3 was predicted and has shown three conserved domains: an (α/β)8 triose phosphate isomerase (TIM) barrel, (α/β)5 sandwich, and fibronectin type III domain involved in protein thermostability. Zymogram analysis highlighted such thermostable character of this novel β-glucosidase.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name><name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000183 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000183 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23242634
|texte= Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23242634" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |